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Dang, you beat me to the punch on this one, Ross.
TL;DR
-Short subject lines (the shorter, the better) result in the most opens and click-throughs
-Mild profanity motivates action (hell yeah), but loses effectiveness quickly
-The loyalists (those who are unsubscribe "inelastic") have an extremely high tolerance for emails - you can keep emailing them many times over without an unsubscribe. (I think these email marketing scientists might have read Dan Zarrella's email marketing data: http://www.slideshare.net/HubSpot/the-science-of-email-marketng)